Innovative Longevity Techniques & Protocoles

Our mission is to demonstrate statistically-significant human age reversal so that an eruption of charitable and capitalistic forces will compete to induce even longer, healthier lifespans. We live in an era whereby limitations on maximum lifespans are likely to be soon vanquished. Each day our research is delayed, we grow older and frail. There is tremendous urgency to move human rejuvenation projects forward. Funding has been secured for some clinical studies. The costs of certain projects, however, will require them to be self-funded. In these cases, each study subject will have to pay their portion of expenses of being part of the study.
The dasatinib/quercetin study of senolytics therapy will commence shortly and funding was provided by a long-time Life Extension Foundation supporter. It is divided into three phases to test different dose timing. Phase One is now fully enrolled, but other phases are still enrolling subjects. Participants can travel to Los Angeles or Idaho and must be available for two weekends in a row.
The NAD+ infusion study has commenced and funding has been secured to cover 100% of this study’s cost. This study is fully enrolled. Future studies that will test NAD+ infusions for Parkinson’s, Alzheimer’s, and stroke patients, are being planned. Let us know if you’d like to participate. The rapamycin study site has been moved to Southern California. Funding has been secured to cover 100% of this study’s cost. The primary cost of this and some other studies are the extensive clinical and biomarker measures that must be done to assess if biological age reversal is occurring. Enrollment for this study is currently open.
The GDF11 trial is planned to initiate in Nassau, Bahamas around October of this year and will require each study participant to self-fund $7,800 for one year’s treatment, which includes costs of extensive clinical and biomarker measurements. A clinical trial studying the immunomodulatory properties and cost-effectiveness of mesenchymal stem cells as an alternative treatment for chronic autoimmune conditions is commencing.
The thymus regeneration study will be based in California but is available nationwide. The cost to participate in a one-year trial will be a maximum of $28,000, which includes medications, MRI scans of the thymus (optional), and high-tech monitoring of immune status. The expected re-growth of the thymus gland (based on preliminary results from a 10-patient pilot study) may provide immune restoration benefits.
Young plasma transfer studies (also called Therapeutic Plasma Exchange) will initially be conducted at several sites in Florida, North Carolina, Colorado and Southern California. We anticipate more sites in the U.S. later this year. Two treatment protocols will be offered, one using 5% albumin and immune senescence markers will be offered in both protocols. The expected cost for six infusions including comprehensive measurements of possible efficacy is estimated at $50,000.

 

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Our private association will seek to rescue aged humans who may not live long enough for rejuvenation therapies to be fully validated. We might save many lives, as did the civic-minded Amsterdam group 250 years ago.
Here is the game plan:
1 We believe aging is at least partially reversible using existing therapies;
2 There is a growing interest in transforming this into clinical reality;
3 Those interested in age reversal want active (not passive) engagement;
4 The most efficient way to advance age-reversal research is via a private association (analogous to the Amsterdam society).
Choosing a Name
I initially proposed our private group be named Society for Rescue of Senescent Persons.
Others in the group suggested something more recognizable, so the name on our website is now:
Society for Rescue of Aged Persons

1) Aspirin

Age-related diseases and general aging are often caused by chronic inflammation. In animal studies, using aspirin to quell inflammation resulted in extended lifespans, more physical capability and increased stress resistance [RR].

Aspirin increased the lifespan of mice by 18-21% at doses of 400mg/kg [R].

One study surprisingly found that aspirin was more effective at increasing maximum lifespan than metformin (see below) [R].

The high doses used in these studies might lead to serious intestinal problems [R].

2) C60

French biologists found that rats given C60 (a fullerene molecule), dissolved in olive oil at doses of 1.7 mg/kg of body weight, lived nearly twice as long as control groups and had a reduction of age-related diseases, even though the rats were a middle age at the start of the study. Rats have never lived as long in any study [R].

None of the rats treated with C60 got cancer, so studies looking at C60 and cancer prevention are currently underway [R].

The longevity mechanisms of C60 are currently not fully understood, but C60 is known to be bioactive in a number of ways, such as affecting DNA expression and protein folding [R].

C60 easily crosses the blood-brain barrier. It concentrates in the mitochondria where it functions as a free radical scavenger i.e. it detoxifies the by-products from the cell’s energy metabolism [R].

C60 molecules have the potential to block the replication of the HIV-1 virus so could prove to be an important weapon against AIDs [R].

C60 has never been tested on humans.

3) Lithium

Many people think that the most effective anti-aging strategies start by addressing the mitochondria.

Lithium treatment on worms increased both lifespan and healthspan and improved mitochondrial energy output [R].

Lithium might improve mitochondrial function by increasing the turnover of dysfunctional mitochondria [R].

However, a recent study of flies found the opposite outcome: lithium exposure did not extend lifespan and actually reduced the female fly’s lifespan advantage [R].

A correlation was found when researchers measured the longevity of people and lithium in their water.  There was a decreased risk for all causes of death in Japanese neighborhoods with higher lithium levels.

The study concluded that “long-term low-dose exposure to lithium may exert anti-aging capabilities and unambiguously decreases mortality in evolutionary distinct species” [R].

One mechanism might be that lithium should theoretically increase NAD+, which is associated with longevity.

(Lithium inhibits mir-34a, which in turn inhibits NAMPT, the enzyme that makes NAD+ [R, R]. Therefore, lithium probably increases NAD+.)

4) Curcumin

Curcumin increased the median and maximum lifespan of flies by up to 25.8%. The optimal dose of curcumin for male flies (0.5mg/g of diet) was lower than for females (1mg/g of diet) [R].

Lifespan extension with curcumin has been attributed to its ability to decrease expression of age-related genes (including mTOR).

Curcumin supplementation also acts as a potent antioxidant in the cell [R, R].

The increase in lifespans did not change if the flies were also calorie restricted, suggesting that curcumin and caloric restriction act on the same biological pathways [R].

I find that curcumin is the most multifunctional supplement available. But not all curcumin supplements are created equal. Many are not bioavailable and do not reach the brain.y.

5) Oxaloacetate

Oxaloacetate supplementation increased lifespan in worms by stimulating the same longevity pathways as calorie restriction [R].

Accumulation of proteins is a common cause of aging. Oxaloacetate might increase lifespan by reducing the build-up of methylglyoxal (MG) – an important source of protein toxicity and general biological dysfunction [R].

A build-up of glutamate can be toxic to cells. It causes serious brain damage after a concussion or stroke and is the favorite energy source for many cancers [R, R].

Consumption of oxaloacetate can lower blood glutamate levels by 40%. Rats given oxaloacetate had a 237% increase in brain tumor survival rates [R].

Oxaloacetate also increases NAD+.

Oxaloacetate is a cellular metabolite and so is not available from a dietary source. It must be taken in supplement form.

I recommend taking it in the morning or afternoon with meals.  For me to notice a benefit, I need to take 3 pills a day, but since it’s expensive, I take one daily.

Oxaloacetate

6) Rhodiola

Rhodiola rosea is an adaptogenic herb commonly used to improve stress resilience.

R. rosea can extend lifespan in flies, worms, and yeast [R].

Rhodiola was shown to extend lifespan in both sexes regardless of diet, suggesting that it works on different longevity pathways to caloric restriction [R].

Rhodiola is a supplement that I like to take frequently.  There are many options, but I do better when the rhodiola’s that have a higher salidroside percentage.

Here are the best options:

Rhodiola or Rhodiola

7) Carnitine

Mitochondrial decay is a significant factor in aging, caused, in part, by the release of reactive oxygen species (ROS) as by-products of mitochondrial electron transport.

Supplementing with carnitine can improve mitochondrial health and efficiency by enhancing electron flow [R].

In one study supplemental carnitine prolonged the aging of yeast cells [R].

The increased electron flow from carnitine also increases the formation of reactive oxygen species. It might be a wise idea to take an antioxidant, such as lipoic acid, along with carnitine [R].

•Buy: Acetyl L Carnitine

8) NAC

N-acetylcysteine (NAC) is a modified sulfur-containing amino acid that acts as a potent cellular antioxidant [R].

NAC extended the lives of flies. Flies fed NAC lived 26.6% longer than normal [R].

Similarly, the lifespan of worms was extended by up to 30.5% with supplemental NAC. The same study showed that NAC significantly increased resistance to a variety of environmental stressors [R].

Because NAC is an antioxidant it may slow the aging process by protecting the organism against free radical-induced damage [R].

NAC effects on longevity might also involve its impact on the expression of specific mRNA genes [R].

NAC is widely available in supplemental form. I suggest capsules as loose crystals taste disgusting.

•I recommend  NAC

9) Carnosine

Carnosine is an antioxidant and has been shown to reduce the brain damage from oxidative stress [R].

Male flies that were given carnosine lived on average 20% longer than normal. No increase in lifespan was noted in female flies taking carnosine [R, R].

I recommend taking this right upon awakening or a half hour before food.  Carnosine binds to divalent metals like magnesium and calcium and others, so you don’t want to take this with meals.

•Buy: Carnosine

10) Melatonin

Melatonin is an important regulator of circadian rhythms and is a potent anti-inflammatory compound [R].

Increases in the longevity of fruit flies, mice and rats have been observed when melatonin was given supplementally or added to their food [R, R, R].

In both mice and rats, melatonin acts as a potent antioxidant and inhibits free radical damage [R].

Melatonin has effects on the expression of genes that govern the cell cycle, cell/organism defense, protein expression and transport, and mitochondrial function. It might also activate the same sirtuin pathways as caloric restriction (SIRT1) [R].

One of the best ways to increase melatonin is to minimize exposure to artificial light after sunset [R].

There are many types of melatonin, and people will differ in which kind works for them.  Here are some interesting types of melatonin.

•Buy: Melatonin Cream, Melatonin Drops, Melatonin-time release,  Melatonin

11) Lactic acid

Feeding lactic acid to fruit flies increased median lifespan by 12-15% depending on what stage of life supplementation began [R].

It is proposed that lactic acid increases lifespan by removing hydroxyl radicals [R]..

12) Gluconic Acid

Gluconic acid increased the lifespan of flies by 12-22% depending on what stage of life supplementation began [R].

In another study dietary gluconic acid slowed the normal age-related accumulation of copper in adult flies and lead to an increased lifespan of 21.6% [R].

Like lactic acid, gluconic acid increases lifespan by removing hydroxyl radicals [R].

Kombucha is a good source of gluconic acid.

Potassium gluconate is a good form of potassium and gluconic acid.

Potassium Gluconate Powder

13) NAD+

Supplemental NAD led to an extension of lifespan in yeast by activating SIRT1 [R].

Niagen is a good way to increase NAD levels:

Nicotinamide Riboside – Niagen NAD

This is a relatively new supplement, but my guess is it’s going to become more popular because of its noticeably powerful effect on improving energy and mitochondria.  Its only downside is its expense.

14) Malate

Malate was found to extend the lifespan and stress tolerance in worms through activation of gene pathways that code for longevity (DAF-16 and SIRT1) [R].

Malate did not extend the lifespan of worms that were also calorie restricted.

Malate can be synthesized from fumarate. Hence, taking fumarate also extends lifespan [R].

Malic acid is also good for chelating heavy metals and to increase energy production.

Malic Acid

16) Acetate

Acetic acid increases the lifespan of worms (by increasing DAF-16). This extension of lifespan was 30-40% greater when acetic acid was combined with Reishi extract [R].

Vinegar is a good source of acetic acid.

Apple Cider Vinegar

17) Pyruvate

Worms with the slcf-1 gene mutation had increased blood pyruvate levels and a 40% increase in lifespan [R].

There are Pyruvate supplements that you can take.

18) Activated charcoal

Activated charcoal is able to absorb substances from the digestive tract. It can be used to eliminate toxic substances and can alter the fat and protein content of the gut.

Rats were given an activated carbon compound live an average of 37-60% longer. By absorbing toxic substances in the digestive tract carbon was found to delay age-related structural changes in the organs and tissues [R, R].

Here is the source I often recommend:

Activated Charcoal

19) Lutein

Lutein is an abundant carotenoid in fruits and vegetables.

Lutein prolonged the average lifespan of fruit flies by 63% [R].

Lutein extends lifespan by increasing antioxidant enzyme activity and up-regulating the expression of certain genes that correspond to longevity (SOD1, SOD2 & CAT) [R].

You can get lutein by eating lots of fruits and veg, but I also like to supplement.

20) Theaflavins

The lifespan of flies was extended by approximately 10% when given a supplemental black tea extract rich in theaflavins. The flies given BTE also showed an increased resistance to the negative effects of a high-fat diet [R].

The longevity-enhancing effects of BTE are likely controlled, at least in part, by its impact on the gene expression of SOD and CAT [R].

I find this one by Life Extension to be effective:

Theaflavins

21) Inositol

D-chiro-inositol slowed the aging process and enhanced longevity in flies [R].

High levels of blood glucose inhibit cell growth and encourage cell aging. Myoinositol protects against the damage done by high glucose levels [R].

D-chiro-inositol/Pinitol is found in carob.

I use 5g per a day of inositol.  These are good options:

Inositol

D-Chiro Inositol

Carob

22) Metformin

Metformin belongs to a family of drugs called biguanides that have been shown to increase lifespan. For example, Metformin extended the lifespan of worms by 40% (median) and significantly increased the lifespan of mice [R].

Like many other life-extending drugs, metformin induces many of the benefits of calorie restriction, such as improved physical performance, increased insulin sensitivity, and reduced LDL and cholesterol levels without actual calorie restriction [R].

Metformin might extend lifespan by behaving as a metabolic stressor that activates an oxidative stress and detoxification response (involving AMPK) [R, R, R].

In animal models, deactivation of IGF-1 genes increases lifespan. Metformin decreases IGF-1 [R].

As already mentioned, blood sugar regulation is an important factor in aging. Metformin lowers blood sugar by increasing insulin sensitivity in liver and muscles. It might also work by suppressing glucose production in the liver [R].

Metformin mitigates the high risk of diabetics getting cardiovascular disease and brain issues.

Diabetics who take metformin are at decreased risk of developing a range of cancers. It is unclear whether these cancer prevention properties would apply to non-diabetics [R, R].

Metformin changes the microbiome in a way that promotes health and longevity, possibly by altering microbial Folate and Methionine Metabolism [R, R].

Metformin promotes mobility in old age, decreases the fat build up,  and increases stress resilience in response to oxygen deprivation [R].

Metformin is a prescription drug, so speak with your doctor before you take it.

23) Deprenyl – MAOBI

Deprenyl’s positive effects on longevity have been tested in at least five different animal species by independent research groups.

Rats treated with .25mg/kg of deprenyl lived an average of 30% longer than the control groups [R, R].

Deprenyl given at 1 mg/kg significantly prolonged the lifespan of middle-aged dogs when taken for a minimum of six months [R].

Deprenyl might increase longevity by raising antioxidant enzymes such as superoxide dismutase (SOD) and catalase (CAT) in certain brain regions [R].

24) Butyrate

Butyrate is a short chain fatty acid produced during fermentation by the gut microbiota.

Feeding a form of butyrate to flies increased their maximum lifespan by 30-50% [R].

Similar life extension properties of butyrate have been observed in worms [R].

One of the primary mechanisms by which Butyrate extends lifespan is by altering the copying of genes that code for longevity (e.g glutathione S-transferase & superoxide dismutase) [R].

Internal Butyrate levels can be increased via supplementation, or by eating more resistant starch and non-digestible fibers that encourage colonic fermentation.

Butyrate

•Hi-Maize resistant starch at 50g/day to start

25) Glucosamine

In worm and mouse studies, glucosamine has extended lifespan through creating new mitochondria [R].

In people, glucosamine supplements were associated with a lower risk of dying [R].

Glucosamine has an inhibitory effect on tumors [R].

•Buy: Glucosamine

26) Resveratrol

Resveratrol has been found to increase lifespan in yeast, worms, flies, bees, fish, and rodents [R].

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